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Evaluation of slide agglutination methods for identifying group D streptococci
Journal of Clinical Microbiology
|May 1, 1983
Summary
Evaluating commercial reagents for group D streptococci identification, SeroSTAT and Streptex showed 90% accuracy with pronase extraction. SeroSTAT offered better specificity, proving useful for rapid clinical laboratory diagnosis.
Area of Science:
- Clinical Microbiology
- Bacteriology
- Immunodiagnostics
Background:
- Group D streptococci are significant human pathogens.
- Rapid and accurate identification is crucial for timely clinical management.
- Commercial slide agglutination reagents offer potential for rapid diagnostics.
Purpose of the Study:
- To evaluate the performance of three commercial slide agglutination reagents for identifying group D streptococci.
- To compare different testing methods: direct colony, enzyme extraction (pronase), and broth culture.
- To assess the specificity and sensitivity of each reagent.
Main Methods:
- Evaluation of SeroSTAT, Streptex, and Phadebact reagents.
- Testing of 72 strains of group D streptococci.
- Comparison of direct colony, pronase extraction, and broth culture methods.
Main Results:
- SeroSTAT and Streptex, using pronase extraction, identified 90% (65/72) of group D streptococci strains.
- SeroSTAT demonstrated higher specificity, with no cross-reactivity observed with viridans group streptococci.
- The Phadebact reagent yielded non-reactive results.
Conclusions:
- Latex agglutination reagents, particularly SeroSTAT and Streptex with pronase extraction, are valuable tools for rapid identification of group D streptococci.
- SeroSTAT offers a good balance of sensitivity and specificity for clinical laboratory use.
- These methods facilitate quick recognition of group D streptococci, aiding clinical decision-making.