The evolution of an area centralis and visual streak in the marsupial Setonix brachyurus

The distribution, morphology, size, and number of cells in the retinal ganglion layer of the marsupial Setonix brachyurus, "quokka," was studied from 25 days postnatal to adulthood using Nissl-stained wholemounts. The total cell population was evenly distributed up to 50 days, but by 75 days highest densities were generally observed in a broad band extending across the nasotemporal axis. At 87 days, a temporally situated area centralis was seen for the first time. This was embedded in a horizontally aligned visual streak, the nasal arm of which contained areas of high density. By 106 days, densities in the area centralis had stabilized while peripheral values were higher than adult levels even at 180 days. In the adult, the area centralis was surrounded by a weak visual streak. Retinal area increased steadily during development to reach 168 mm2 at 180 days, the adult range being 225-250 mm2. All cells in the ganglion layer appeared undifferentiated and rounded at 33 days with soma diameters of 3-6 micrometers; by 70 days diameters had increased to 4-12 micrometers and some cells had axon hillocks containing Nissl substance. From 87 days we distinguished ganglion cells, which constituted 54-63% of the total. These were identified by deeply stained Nissl substance and had diameters of 7-18 micrometers, compared to 7-23 micrometers at 143 days and 7-24 micrometers in the adult; the remaining cells, termed glia/interneurons, were 5-8 micrometers throughout. Only ganglion cells were organized into an area centralis and visual streak. Glia/interneurons were evenly distributed except at the extreme periphery, where their density increased. In sectioned material, the ganglion layer was distinct from 25 days while the neuroblastic layer separated only between 48 and 85 days. From 25 to 250 days the total number of cells in the ganglion layer remained similar to the adult range of 336,000-393,000. At both 87 days and in adults optic axon counts fell between 180,000 and 224,000, close to ganglionic cell estimates. At 25 and 34 days, respectively, optic axon numbers were 75,000 and 172,000. Myelination was absent at 25 and 34 days, 3% at 87 days, and almost 100% in adults. Mechanisms are discussed whereby the area centralis and visual streak may evolve from an even distribution of cells while their number remains constant; migration is considered likely to be important.