Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Complement receptor mediates enhanced flavivirus replication in macrophages.

M J Cardosa, J S Porterfield, S Gordon

    The Journal of Experimental Medicine
    |July 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Enterovirus 71 encephalomyelitis and Japanese encephalitis can be distinguished by topographic distribution of inflammation and specific intraneuronal detection of viral antigen and RNA.

    Neuropathology and applied neurobiology·2012
    Same author

    Novel multiplex oligonucleotide-conjugated bead suspension array for rapid identification of enterovirus 71 subgenogroups.

    Journal of clinical microbiology·2010
    Same author

    High-titred neutralizing antibodies to human enterovirus 71 preferentially bind to the N-terminal portion of the capsid protein VP1.

    Archives of virology·2007
    Same author

    Molecular phylogeny of modern coxsackievirus A16.

    Archives of virology·2007
    Same author

    Review on flavivirus vaccine development. Proceedings of a meeting jointly organised by the World Health Organization and the Thai Ministry of Public Health, 26-27 April 2004, Bangkok, Thailand.

    Vaccine·2005
    Same author

    Mary Jane Cardosa--bringing local solutions to Sarawak, Malaysia.

    The Lancet. Infectious diseases·2002

    Complement receptors (CR3) on macrophages enhance flavivirus replication when IgM antibodies are present. This enhancement is specifically blocked by targeting CR3, not Fc receptors, indicating a key role for CR3 in flavivirus infection.

    Area of Science:

    • Immunology
    • Virology
    • Cell Biology

    Background:

    • Flavivirus infections pose significant global health challenges.
    • Macrophage complement receptors play a role in immune responses.
    • Antibody-dependent enhancement (ADE) can exacerbate viral infections.

    Purpose of the Study:

    • To investigate the role of macrophage complement receptors in IgM-dependent flavivirus replication.
    • To determine whether complement receptors (CR3) or Fc receptors mediate this enhancement.

    Main Methods:

    • Utilized macrophages and flavivirus in cell culture experiments.
    • Employed monoclonal antibodies (mAb Ml/70 and mAb 2.4G2) to block specific receptors.
    • Assessed flavivirus replication levels following antibody treatments.

    Related Experiment Videos

    Main Results:

    • Macrophage complement receptors type 3 (CR3) mediate IgM-dependent enhancement of flavivirus replication.
    • Pretreatment with mAb Ml/70, which inhibits CR3 binding, blocked this enhancement.
    • Pretreatment with mAb 2.4G2, which inhibits Fc receptor binding, did not block the enhancement.

    Conclusions:

    • CR3, not Fc receptors, is the primary mediator of IgM-dependent enhancement of flavivirus replication in macrophages.
    • Targeting CR3 may offer a novel therapeutic strategy for flavivirus infections.