In vivo acetylcholine turnover in rat heart

The in vivo uptake of choline (Ch) and synthesis of acetylcholine (ACh) in rat heart were studied using a pyrolysis mass fragmentography (PMF) method. Deuterium labeled Ch was pulse injected (i.v.) into anesthetized rats. Labeled and unlabeled Ch and ACh were measured by PMF in hearts at various times following injection. From these data we calculated the specific activities of Ch and ACh, rate constants for ACh and turnover rates of ACh. After an initial equilibration period of approximately 2 min, the specific activities of Ch and ACh decayed in a parallel manner with half-times of 28.2 and 28.8 min respectively. Between 2 and 60 min the calculated ACh turnover rate was 0.144 nmol/g/min. Unlike brain Ch, heart Ch levels are very stable with time following sacrifice. No advantage was found in using microwave irradiation to stabilize heart ACh and Ch content.