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[Argasid tick lysozyme action on HEp-2 cells].
Antibiotiki
|August 1, 1978
Summary
Argasidae lysozyme exhibits cytotoxic effects on HEp-2 cells at high concentrations, while lower doses and FL cells show no sensitivity. Egg lysozyme demonstrates similar cytotoxicity at elevated levels.
Area of Science:
- Biochemistry
- Cell Biology
- Parasitology
Context:
- Investigating the biological activity of lysozymes from different sources.
- Evaluating the cytotoxic potential of Argasidae lysozyme, derived from Ornithodoros papillipes.
- Comparing the effects of Argasidae lysozyme with traditional egg lysozyme on cell lines.
Purpose:
- To determine the cytotoxic effects of Argasidae lysozyme on HEp-2 and FL cell lines.
- To compare the cytotoxic activity of Argasidae lysozyme with egg lysozyme.
- To assess the impact of these lysozymes on cellular mitotic activity and DNA synthesis.
Summary:
- Argasidae lysozyme demonstrated cytotoxic effects on HEp-2 cells at concentrations of 300 and 500 µg/ml, with no observed activity at 50 or 100 µg/ml.
- FL cells were insensitive to the tested concentrations of Argasidae lysozyme.
- Egg lysozyme exhibited analogous cytotoxic effects on HEp-2 cells at concentrations of 500 µg/ml and higher.
- Neither Argasidae nor egg lysozyme significantly affected the mitotic activity or DNA synthesis in the treated cell lines, as indicated by cariogramme analysis and 3H-thymidine incorporation.
Impact:
- Provides insights into the differential cytotoxicity of lysozymes from various biological origins.
- Highlights the potential of Argasidae lysozyme as a subject for further toxicological and biological studies.
- Establishes a baseline understanding of lysozyme's effects on specific cell lines, crucial for potential therapeutic or diagnostic applications.