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Related Experiment Videos

Kidney preservation failure using combined Collins' solution and plasma perfusion.

R T Schweizer, B A Sutphin

    Transplantation
    |July 1, 1983
    PubMed
    Summary

    High magnesium levels in cryoprecipitated plasma (CPP) can cause clotting, impacting organ preservation. Careful consideration of magnesium concentration is crucial when using CPP for perfusion preservation, especially after certain flushing solutions.

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    Area of Science:

    • Biomedical Engineering
    • Organ Transplantation
    • Biochemistry

    Background:

    • Cryoprecipitated plasma (CPP) is used in organ preservation.
    • High magnesium concentrations can interfere with anticoagulation.
    • Fibrin formation is a critical issue in organ perfusion.

    Purpose of the Study:

    • To investigate the effect of magnesium levels in flushing solutions on CPP anticoagulation.
    • To determine the risk of fibrin formation during kidney perfusion with CPP.

    Main Methods:

    • Pig cadaver kidneys were flushed with Collins' C2 solution with varying magnesium levels.
    • Kidneys were subsequently perfused with CPP.
    • CPP perfusate was analyzed for fibrin formation.
    • Alternative flushing and preservation methods were tested.

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    Main Results:

    • High magnesium in Collins' C2 solution led to CPP perfusate clotting.
    • Low magnesium levels in the flushing solution did not cause clotting.
    • Heparin addition did not prevent magnesium-induced fibrin formation.
    • Alternative preservation methods (albumin, Ringer's solution) avoided clotting.

    Conclusions:

    • Collins' C2 solution with high magnesium is incompatible with CPP perfusion.
    • Magnesium levels must be carefully controlled to prevent fibrin formation during CPP perfusion.
    • Kidney preservation protocols require reassessment when combining C2 flushing with CPP perfusion.