Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cord blood platelet aggregation; quality control by a two-sample technique.

G Ahlsten, U Ewald, T Tuvemo

    Upsala Journal of Medical Sciences
    |January 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Simulation-based team training improved the self-assessed ability of physicians, nurses and midwives to perform neonatal resuscitation.

    Acta paediatrica (Oslo, Norway : 1992)·2017
    Same author

    Improved postnatal care is needed to maintain gains in neonatal survival after the implementation of the Helping Babies Breathe initiative.

    Acta paediatrica (Oslo, Norway : 1992)·2017
    Same author

    Ophthalmological outcome and visuospatial ability in very preterm children measured at 2.5 years corrected age.

    Acta paediatrica (Oslo, Norway : 1992)·2013
    Same author

    Cytomegalovirus-specific CD4 and CD8 T cell responses in infants and children.

    Scandinavian journal of immunology·2012
    Same author

    State of the art and recommendations. Kangaroo mother care: application in a high-tech environment.

    Breastfeeding review : professional publication of the Nursing Mothers' Association of Australia·2011
    Same author

    The influence of ICT on the activity patterns of children with physical disabilities outside school.

    Child: care, health and development·2010

    To reduce variability in cord blood platelet aggregation, collect two samples within 8 minutes of birth. Ensure platelet-rich plasma counts have less than a 5% difference for consistent results.

    Area of Science:

    • Hematology
    • Neonatal Research
    • Platelet Physiology

    Background:

    • Cord blood is a valuable source for neonatal hematological studies.
    • Platelet aggregation is crucial for hemostasis but can be variable in cord blood samples.
    • Standardizing collection methods is essential for reliable platelet function testing.

    Purpose of the Study:

    • To investigate the impact of sample timing on cord blood platelet aggregation.
    • To identify methods for reducing variability in platelet aggregation assays.
    • To establish criteria for acceptable cord blood sample pairs for aggregation studies.

    Main Methods:

    • Collected two cord blood samples (2-4 min and 5-8 min post-birth) from 17 normal deliveries.
    • Assessed platelet counts in platelet-rich plasma (PRP) and performed platelet aggregation studies.

    Related Experiment Videos

  • Compared results between early and late samples, categorizing cords into Group A (<5% count difference) and Group B (>5% count difference).

    • Main Results:

    • Group A showed consistent aggregation results and high correlations between early and late samples.
    • Group B exhibited inconsistent aggregation results between the two sample timings.
    • Late samples in Group A demonstrated slightly higher aggregation responses (p<0.01).
    • Acid-base differences did not explain the observed variations.

    Conclusions:

    • Platelet aggregation variability in cord blood can be significantly reduced by selecting samples with concordant PRP platelet counts (<5% difference).
    • This standardization method enhances the reliability of neonatal platelet function testing.
    • Utilizing paired samples with minimal platelet count variation improves the consistency of cord blood platelet aggregation data.