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Related Experiment Videos

Peripheral lymphocyte function in dogs with Brucella canis infection.

C A Johnson, R W Bull, R G Schirmer

    Veterinary Immunology and Immunopathology
    |May 1, 1983
    PubMed
    Summary

    Canine Brucella canis infection impairs peripheral lymphocyte function, specifically reducing responses to PHA mitogen. However, infected dogs

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    Area of Science:

    • Veterinary immunology
    • Canine infectious diseases

    Background:

    • Brucella canis is a significant pathogen causing reproductive disease in dogs.
    • Understanding the immune response in infected dogs is crucial for disease management.

    Purpose of the Study:

    • To evaluate peripheral lymphocyte function in dogs naturally infected with Brucella canis.
    • To compare the in vitro response of lymphocytes from infected and control dogs to mitogens and Brucella canis antigen.

    Main Methods:

    • Peripheral blood lymphocytes were isolated from naturally infected and control bitches.
    • In vitro lymphocyte stimulation assays were performed using phytohemagglutinin (PHA), concanavalin A (Con A), pokeweed mitogen (PWM), and killed Brucella canis organisms.
    • Lymphocyte proliferation was measured as an indicator of immune response.

    Main Results:

    • Lymphocytes from infected dogs showed significantly reduced responsiveness to PHA compared to controls (p < .05).
    • No significant difference in lymphocyte response to Con A and PWM was observed between infected and control groups.
    • Lymphocytes from infected dogs demonstrated a significant proliferative response to Brucella canis antigen, while control lymphocytes did not (p < .005).

    Conclusions:

    • Brucella canis infection alters canine peripheral lymphocyte function, characterized by hyporesponsiveness to PHA.
    • The specific response of lymphocytes to Brucella canis antigen in infected dogs suggests a role for cellular immunity in the disease process.
    • These findings contribute to understanding the immunopathogenesis of Brucella canis infection in dogs.

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