Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Exogenous substrate utilization by isolated myocytes from chronically diabetic rats.

V Chen, G J Bagby, J J Spitzer

    The American Journal of Physiology
    |July 1, 1983
    PubMed
    Summary

    Diabetic rat heart cells show reduced glucose and lactate use but normal palmitate use. Insulin treatment reversed these metabolic changes in diabetic heart cells.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Decreased myoblast differentiation in chronic binge alcohol-administered simian immunodeficiency virus-infected male macaques: role of decreased miR-206.

    American journal of physiology. Regulatory, integrative and comparative physiology·2017
    Same author

    Hemorrhage alters neuroendocrine, hemodynamic, and compartment-specific TNF responses to LPS.

    Shock (Augusta, Ga.)·2002
    Same author

    Increased granulopoiesis through interleukin-17 and granulocyte colony-stimulating factor in leukocyte adhesion molecule-deficient mice.

    Blood·2001
    Same author

    Acute ethanol intoxication suppresses lung chemokine production following infection with Streptococcus pneumoniae.

    The Journal of infectious diseases·2001
    Same author

    Requirement of interleukin 17 receptor signaling for lung CXC chemokine and granulocyte colony-stimulating factor expression, neutrophil recruitment, and host defense.

    The Journal of experimental medicine·2001
    Same author

    Cytokine treatment of bacterial pneumonia.

    Seminars in respiratory infections·2001

    Area of Science:

    • Cardiology
    • Metabolic Research
    • Cellular Physiology

    Background:

    • Chronic diabetes alters cardiac substrate utilization.
    • Understanding these metabolic shifts is crucial for managing diabetic cardiomyopathy.

    Purpose of the Study:

    • To investigate how chronic diabetes affects the use of different energy sources (substrates) by heart cells (myocytes).
    • To examine the impact of substrate concentration on energy metabolism in diabetic rat myocytes.

    Main Methods:

    • Isolated, calcium-tolerant, nonbeating myocytes from streptozotocin-induced diabetic and control rats were used.
    • Rates of glucose, lactate, and palmitate oxidation were measured.
    • Oxygen consumption and cellular energy levels (ATP, phosphocreatine) were assessed.

    Related Experiment Videos

    Main Results:

    • Diabetic myocytes showed reduced oxidation of glucose and lactate but maintained palmitate oxidation compared to controls.
    • Increased substrate concentrations in vitro partially normalized substrate utilization in diabetic myocytes.
    • Diabetic myocytes exhibited lower oxygen consumption with lactate or palmitate, despite similar ATP and phosphocreatine levels.
    • In vivo insulin treatment reversed all observed metabolic alterations in diabetic myocytes.

    Conclusions:

    • Chronic diabetes impairs cardiac myocyte utilization of glucose and lactate, while palmitate oxidation remains relatively preserved.
    • These metabolic dysfunctions are reversible with insulin therapy, highlighting the importance of glycemic control in diabetic heart disease.