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Updated: Aug 14, 2026

A Decentralized (Ex Vivo) Murine Bladder Model with the Detrusor Muscle Removed for Direct Access to the Suburothelium during Bladder Filling
Published on: November 28, 2019
Control of ion transport by Gillichthys mirabilis urinary bladder
Abstract:
The columnar epithelial cell region of the goby Gillichthys mirabilis urinary bladder is the region responsible for active Na and Cl reabsorption from bladder urine. In 5% seawater-adapted fish, reabsorption occurs via an electrically silent coupled NaCl transport mechanism. Bladder reabsorption is increased in seawater-adapted fish above that observed in 5% seawater-adapted fish; incremental reabsorption results from an electrogenic Na transport in addition to the neutral component. Hypophysectomy of seawater-adapted fish reduces the electrogenic Na transport (measured as short-circuit current, ISC) and increases the transepithelial resistance (R) to values near those of 5% seawater-adapted fish. Cortisol restores the ISC and R to normal seawater-adapted values and will initiate electrogenic Na transport in 5% seawater-adapted fish. Ovine prolactin will also restore the ISC and R of columnar cell regions of hypophysectomized seawater-adapted fish to normal seawater-adapted levels; this effect appears to be due to contamination or to inherent stimulatory activity of the ovine prolactin preparation, since endogenous prolactin is ineffective in the restoration of ISC or R.
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