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Methane oxidation by Nitrosomonas europaea.

M R Hyman, P M Wood

    The Biochemical Journal
    |April 15, 1983
    PubMed
    Summary

    Methane acts as an alternative substrate for ammonia mono-oxygenase in Nitrosomonas europaea, impacting ammonia utilization and producing methanol. This reveals a novel metabolic pathway for this key ammonia-oxidizing bacterium.

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    Area of Science:

    • Microbiology
    • Biochemistry
    • Environmental Science

    Background:

    • Nitrosomonas europaea is a key ammonia-oxidizing bacterium in nitrification.
    • Ammonia mono-oxygenase (AMO) is the primary enzyme responsible for ammonia oxidation.

    Purpose of the Study:

    • To investigate the interaction between methane and ammonia oxidation in Nitrosomonas europaea.
    • To determine if methane can serve as a substrate for ammonia mono-oxygenase.

    Main Methods:

    • Enzyme kinetics studies using varying methane and ammonia concentrations.
    • Oxygen consumption assays.
    • Methanol production measurements.
    • Inhibition studies with allylthiourea.

    Main Results:

    • Methane inhibited ammonia utilization by Nitrosomonas europaea with a Ki of 2mM.
    • Oxygen consumption was not inhibited by methane; instead, it was stimulated in the absence of ammonia.
    • Methanol was detected as a product, with yields up to 1 mol methanol per mol O2 consumed.
    • Methane's stimulatory effect on oxygen uptake was abolished by allylthiourea, an inhibitor of ammonia oxidation.

    Conclusions:

    • Methane is an alternative substrate for ammonia mono-oxygenase in Nitrosomonas europaea.
    • This interaction has implications for the nitrogen cycle and microbial metabolism.

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