Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Acetal phosphatidic acids: novel platelet aggregating agents.

J P Brammer, M H Maguire, E J Walaszek

    British Journal of Pharmacology
    |May 1, 1983
    PubMed
    Summary

    Acetal phosphatidic acids potently induce platelet aggregation, with palmitaldehyde acetal phosphatidic acid being most effective. These compounds may play a role in physiological or pathological processes.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Some biologically active n(6)-methylated adenosine analogs.

    Journal of medicinal chemistry·2011
    Same author

    Simultaneous measurement of adenosine and hypoxanthine in human umbilical cord plasma using reversed-phase high-performance liquid chromatography with photodiode-array detection and on-line validation of peak purity.

    Journal of chromatography. B, Biomedical sciences and applications·1998
    Same author

    Evidence for a novel P2X purinoceptor in human placental chorionic surface arteries.

    European journal of pharmacology·1997
    Same author

    On-line recognition and quantitation of coeluting hypoxanthine and guanine in reversed-phase high-performance liquid chromatography of placental tissue extracts: photodiode-array detection and spectral analysis of coeluting peaks.

    Analytical biochemistry·1993
    Same author

    Peptidomimetics derived from natural products.

    Medicinal research reviews·1993
    Same author

    Effect of preparation techniques on mixing of additives in intravenous fluids in nonrigid containers.

    Hospital pharmacy·1993

    Area of Science:

    • Biochemistry
    • Pharmacology
    • Hematology

    Background:

    • Platelet aggregation is crucial for hemostasis and thrombosis.
    • Phospholipids play diverse roles in cellular functions.
    • The platelet-aggregating properties of certain phospholipids are not fully understood.

    Purpose of the Study:

    • To investigate the platelet-aggregating activity of acetal phosphatidic acids.
    • To determine the potency and mechanism of action of these compounds.
    • To explore their potential physiological or pathological significance.

    Main Methods:

    • Platelet-rich plasma aggregation assays using human and sheep platelets.
    • Dose-response studies to determine threshold concentrations and potency.
    • Investigation of platelet shape change and release of serotonin (5-HT).

    Related Experiment Videos

  • Use of pharmacological inhibitors (e.g., aspirin, 2-methylthio-AMP) and other agents (e.g., chlorpromazine, mepacrine, PGE1).

    • Main Results:

    • Acetal phosphatidic acids, particularly palmitaldehyde acetal phosphatidic acid (PGAP), induced dose-dependent platelet aggregation.
    • Aggregation was biphasic (reversible at low doses, irreversible at high doses) in humans and monophasic in sheep.
    • PGAP was significantly more potent in human platelets compared to olealdehyde and linolealdehyde congeners.
    • Irreversible aggregation was associated with serotonin release in human platelets, but not reversible aggregation.
    • Inhibitors affected different phases of aggregation and release, suggesting distinct mechanisms.
    • Related phospholipids like lecithin and phosphatidic acid did not induce aggregation, while 1-palmityl lysophosphatidic acid showed weak activity.

    Conclusions:

    • Acetal phosphatidic acids directly induce platelet aggregation via interaction with the platelet membrane.
    • The acetal function is critical for their potent platelet-stimulating activity.
    • Given their presence in tissue extracts like 'Darmstoff', these compounds may have significant physiological or pathological roles in platelet function.