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A temperature controller for in vitro recording chambers.

G Rose

    Brain Research Bulletin
    |May 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    This study describes an affordable, low-noise temperature controller for maintaining biological temperatures in tissue slice chambers. Its design ensures reliable in vitro recordings with a clear digital display.

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    Area of Science:

    • Neuroscience
    • Biophysics
    • Physiological Measurement

    Background:

    • Accurate temperature control is crucial for maintaining the viability and function of tissue slices during in vitro electrophysiological recordings.
    • Existing temperature control systems can be expensive, noisy, or prone to errors, impacting experimental reproducibility.

    Purpose of the Study:

    • To describe the design and features of an inexpensive, electrically quiet temperature controller for biological applications.
    • To provide a reliable and robust temperature regulation solution for tissue slice chambers used in in vitro recording.

    Main Methods:

    • Utilized an integrated circuit temperature sensor for linear output and immunity to self-heating.
    • Designed a circuit powered by a single DC supply (12-18 V, 1 A).

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  • Incorporated an output regulator with protection against overheating and short-circuiting.

    • Main Results:

    • The developed temperature controller is cost-effective and operates with minimal electrical noise.
    • Features a linear temperature sensor, robust power supply, and protected output regulator.
    • Includes a digital display for monitoring the preparation's temperature.

    Conclusions:

    • The described temperature controller offers a practical and affordable solution for precise temperature regulation in neuroscience and related research.
    • Its design features enhance reliability and ease of use for in vitro tissue slice experiments.