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Microsampling on the Technicon SMAC system.

T A Walmsley, R T Fowler, M H Abernethy

    Clinical Chemistry
    |August 1, 1983
    PubMed
    Summary
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    Sample volume for the Technicon SMAC analyzer was reduced for routine testing. This optimization enhances efficiency and reduces reagent waste without compromising analytical performance.

    Area of Science:

    • Clinical Chemistry
    • Analytical Chemistry
    • Biomedical Engineering

    Background:

    • The Technicon SMAC continuous-flow analyzer traditionally requires a significant sample volume for comprehensive testing.
    • Reducing sample volume is crucial for improving efficiency and accessibility in routine clinical diagnostics.

    Purpose of the Study:

    • To optimize the Technicon SMAC analyzer for reduced sample volume requirements in routine operation.
    • To maintain or improve analytical performance while decreasing sample input.

    Main Methods:

    • Implemented sequential dialysis for creatinine and iron assays.
    • Increased sample dilution from sixfold to ninefold.
    • Minimized diluted sample stream wastage and enhanced sensitivity for glucose and alkaline phosphatase assays.

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  • Utilized a glycine-containing diluent to improve iron assay sensitivity and prevent protein precipitation.

    • Main Results:

    • Achieved sample volume reduction to 141 microL (17-test profile) or 224 microL (including creatinine and iron).
    • Demonstrated no deterioration in analyzer performance over nine months of routine operation with reduced sample size.
    • Observed a 25% increase in iron method sensitivity with the glycine diluent.

    Conclusions:

    • The optimized Technicon SMAC analyzer effectively reduces sample volume for routine analysis.
    • Reduced sample volume enhances efficiency, decreases calibration material consumption, and facilitates repeat testing without compromising accuracy.
    • The modifications offer a practical advancement for continuous-flow analysis in clinical settings.