Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Genetic polymorphism: from electrophoresis to DNA sequences.

F J Ayala

    Experientia
    |August 15, 1983
    PubMed
    Summary

    Genetic variation is substantial, with most sexually reproducing organisms heterozygous at over 20% of gene loci. DNA sequencing reveals even higher heterozygosity, challenging the neutral theory of molecular evolution.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    GEOGRAPHY OF THE SIBLING SPECIES RELATED TO DROSOPHILA WILLISTONI, AND OF THE SEMISPECIES OF THE DROSOPHILA PAULISTORUM COMPLEX.

    Evolution; international journal of organic evolution·2017
    Same author

    Is Predominant Clonal Evolution a Common Evolutionary Adaptation to Parasitism in Pathogenic Parasitic Protozoa, Fungi, Bacteria, and Viruses?

    Advances in parasitology·2017
    Same author

    Wineries, drosophila, alcohol, and Adh.

    Oecologia·2017
    Same author

    Phylogenetic character mapping of RADES Probing, a new marker for exploring the clonal evolution of expressed coding sequences in Trypanosoma cruzi, the agent of Chagas disease.

    Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases·2013
    Same author

    Climate change and the integrity of science.

    Science (New York, N.Y.)·2010
    Same author

    Using mitochondrial and nuclear markers to evaluate the degree of genetic cohesion among Echinococcus populations.

    Experimental parasitology·2008

    Area of Science:

    • Genetics and Molecular Biology
    • Evolutionary Biology

    Background:

    • Electrophoresis methods underestimate total protein variation.
    • Sexual reproduction leads to significant genetic heterozygosity.

    Purpose of the Study:

    • To assess the extent of genetic variation at the protein and DNA levels.
    • To evaluate the adaptive neutrality of observed genetic variation.

    Main Methods:

    • Analysis of protein variation using electrophoresis.
    • Examination of DNA nucleotide sequences, including introns and exons.

    Main Results:

    • Protein variation undetected by electrophoresis is minimal.
    • High heterozygosity (over 20%) observed at gene loci in sexually reproducing organisms.
    • DNA sequencing indicates near-universal heterozygosity across all loci when considering introns and exons.

    Conclusions:

    • Genetic variation is more extensive than previously estimated by protein electrophoresis.
    • Current evidence does not support the hypothesis of adaptive neutrality for protein-level variation.

    Related Experiment Videos