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A histofluorescent procedure for identifying marijuana cannabinoids.

A Bruni, I Barni Comparini, E Menziani Andreoli

Experientia

|August 15, 1983

Summary

A new fluorescence test, the induced fluorescence identification for marijuana (IFIM) test, reliably identifies glandular hairs in Cannabis sativa. This method offers a more satisfactory way to detect marijuana, cannabis, and hashish in samples compared to the RIM test.

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Area of Science:

Forensic Science
Botany
Analytical Chemistry

Background:

Microscopical identification of Cannabis sativa is crucial in forensic analysis.
Existing methods for identifying marijuana, cannabis, and hashish can be time-consuming or less reliable.
Glandular hairs are key morphological features for Cannabis identification.

Purpose of the Study:

To develop and validate a rapid and reliable fluorescence-based test for microscopical identification of Cannabis sativa glandular hairs.
To introduce the induced fluorescence identification for marijuana (IFIM) test as an alternative to classical methods.
To assess the IFIM test's efficacy in identifying cannabis, hashish, and marijuana in suspected samples.

Main Methods:

A novel fluorescence procedure utilizing a hot clearing solution to induce red fluorescence in cannabinoids.
Microscopical examination of glandular hairs from Cannabis sativa samples.
Comparative analysis of the IFIM test results against the classical RIM test.

Main Results:

The IFIM test successfully induced a distinct red fluorescence in cannabinoids.
The method demonstrated reliability and speed in the microscopical identification of Cannabis sativa glandular hairs.
The IFIM test provided more satisfactory identification of cannabis, hashish, and marijuana compared to the RIM test.

Conclusions:

The IFIM test is a valuable and efficient tool for the forensic identification of marijuana, cannabis, and hashish.
This fluorescence-based method enhances the accuracy and speed of identifying plant material in suspected samples.
The IFIM test offers a promising advancement in forensic botanical analysis.

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