Jove
Visualize
Contact Us

Related Experiment Videos

Photoaffinity labeling: theory and practice.

R J Guillory, S J Jeng

    Federation Proceedings
    |August 1, 1983
    PubMed
    Summary

    Photoaffinity labeling enables in situ reactive reagent generation for studying ligand-binding sites. Despite challenges, this technique is crucial for understanding biological membrane structure-function relationships.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Papain proteolysis releases a soluble NADPH dependent diaphorase activity from bovine neutrophil membranes.

    FEBS letters·1998
    Same author

    Purified leukocyte cytochrome b558 incorporated into liposomes catalyzes a cytosolic factor dependent diaphorase activity.

    Biochemistry·1997
    Same author

    Dietary fat affects lipids and anti-cardiolipin antibody levels in autoimmune-prone NZB/W F1 mice.

    The British journal of nutrition·1997
    Same author

    Dietary fat influences Ia antigen expression, cytokines and prostaglandin E2 production of immune cells in autoimmune-prone NZB x NZW F1 mice.

    The British journal of nutrition·1996
    Same author

    NADPH-binding protein of the neutrophil superoxide-generating oxidase of guinea pigs.

    Proceedings of the National Academy of Sciences of the United States of America·1994
    Same author

    Influences of lovastatin administration on the respiratory burst of leukocytes and the phosphorylation potential of mitochondria in guinea pigs.

    Biochimica et biophysica acta·1994
    JoVE
    x logofacebook logolinkedin logoyoutube logo
    ABOUT JoVE
    OverviewLeadershipBlogJoVE Help Center
    AUTHORS
    Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
    LIBRARIANS
    TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
    RESEARCH
    JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
    EDUCATION
    JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
    Terms & Conditions of Use
    Privacy Policy
    Policies

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Chemical Biology

    Background:

    • Photoaffinity labeling offers unique advantages for studying ligand-binding sites by generating reactive reagents in situ.
    • This method allows labeling of sites lacking inherent reactive nucleophilic residues.
    • Previous studies have highlighted challenges in the application of photoaffinity techniques.

    Purpose of the Study:

    • To examine concerns and challenges associated with the photoaffinity labeling technique.
    • To evaluate the utility of arylazido-beta-alanyl nucleotide probes in photoaffinity labeling.
    • To project the future role of photoaffinity labeling in biological research.

    Main Methods:

    • Utilized arylazido-beta-alanyl nucleotide probes for background studies.
    • Examined concerns and limitations of the photoaffinity labeling approach.
    • Reviewed existing literature and experimental data on photoaffinity labeling.

    Main Results:

    • Photoaffinity labeling provides a special advantage for investigating ligand-binding sites.
    • The technique facilitates the labeling of non-nucleophilic residues.
    • Concerns regarding the application of photoaffinity labeling were systematically addressed.

    Conclusions:

    • Photoaffinity labeling is poised to become a major technique for elucidating structure-function relationships in biological membranes.
    • Future development of photoprobes with shorter activation half-lives will enable studies of time-dependent conformational changes.
    • The technique holds significant promise for advancing our understanding of enzyme active sites and complex ligand-binding sites.

    Related Experiment Videos