Studies on liver specific antigens--partial purification, characterization, and immunization

Seven liver specific antigens, liver protein-I, liver protein-II, liver specific antigen (Espinosa), F-antigen, hepato-renal antigen, liver cell membrane antigen (Ray) and Tamm-Horsfall glycoprotein were partially purified from human liver and urine. Anti-sera against these antigens were raised in rabbits. Anti-sera against liver protein-I, liver protein-II, liver specific antigen (Espinosa) and liver cell membrane antigen (Ray) were absorbed by human serum, kidney, lung and spleen while anti-sera against hepato-renal antigen and Tamm-Horsfall glycoprotein were absorbed by human serum, lung and spleen to remove antibodies against non-specific antigens. There were no cross reactivities among these antibodies.