Comparison between dissociation and inhibition of association of DNA/anti-DNA complexes

A study was made of the increases in ionic strength (mu) and in pH that would effect: a) the inhibition of association of anti-double stranded (ds) DNA and dsDNA, and b) the dissociation of anti-dsDNA from dsDNA, with respect to high avidity as well as to low avidity human anti-dsDNA antibodies, using Crithidia luciliae kinetoplasts as a source of dsDNA. The results obtained appear to indicate that the electrostatic component of the antigen-antibody bond does not become stronger with time (for times spans less than 1 hr).