Least squares fit of data to hyperbolic dose-response curves using a programmed minicalculator (TI-59)

Equations of the Michaelis-Menten form are frequently encountered in a number of areas of biochemical and pharmacological research. A program is presented for use on the programmable TI-59 calculator with added printer which performs an iterative least-squares fit of up to 80 data pairs to this equation and estimates the standard deviations and standard errors of the determined parameters. The program assigns equal weights to errors over the entire data range and is thus appropriate for situations in which data precision is independent of amplitude.