Reversed-phase ion-pair liquid chromatographic procedure with electrochemical detection for the analysis of urinary...

A method using high-performance liquid chromatography with mercury-based electrochemical detection has been developed for the determination of thiosulphate in urine. The chromatographic separation is based upon ion-pair formation between thiosulphate and tetrabutylammonium and reversed-phase chromatography. The method was compared with an earlier reported colorimetric assay and found to be superior with respect to specificity and sensitivity.