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The pentose phosphate pathway in developing chick cornea.

E Masterson, H F Edelhauser, G J Chader

    Biochimica Et Biophysica Acta
    |September 6, 1978
    PubMed
    Summary
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    The pentose phosphate pathway becomes active in embryonic chick corneas during development, coinciding with transparency. This pathway likely generates NADPH for corneal development.

    Area of Science:

    • Developmental biology
    • Biochemistry
    • Ophthalmology

    Background:

    • Corneal transparency is crucial for vision.
    • The pentose phosphate pathway (PPP) is involved in cellular metabolism and biosynthesis.
    • Understanding metabolic changes during embryonic development is key.

    Purpose of the Study:

    • To investigate the activity of the pentose phosphate pathway in embryonic chick corneas during development.
    • To correlate PPP activity with corneal transparency.
    • To determine the functional role of the PPP in corneal development.

    Main Methods:

    • Assessing pentose phosphate pathway activity in embryonic chick corneas at various developmental stages.
    • Measuring glucose uptake.
    • Utilizing phenazine methosulfate as an artificial electron acceptor.

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    Main Results:

    • Pentose phosphate pathway activity appeared at stage 40, coinciding with the onset of corneal transparency.
    • Peak activity was observed at stage 45 and post-hatching.
    • Phenazine methosulfate stimulated PPP activity even before endogenous activity was detectable.
    • No significant increase in glucose uptake was noted.

    Conclusions:

    • Enzymes for the pentose phosphate pathway are present in early embryonic corneas (stage 38-40) but in a latent state.
    • The PPP likely functions to produce NADPH for macromolecular synthesis during corneal development.
    • The pathway's role is primarily NADPH generation, not sugar incorporation.