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Related Experiment Videos

Does the "pacemaker current" generate the diastolic depolarization in the rabbit SA node cells?

A Noma, M Morad, H Irisawa

    Pflugers Archiv : European Journal of Physiology
    |May 1, 1983
    PubMed
    Summary
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    Cesium ions (Cs+) only slightly affect rabbit sino-atrial node pacing despite blocking the inward pacemaker current (ih). The slow inward current (isi) plays a more significant role in pacemaker depolarization.

    Area of Science:

    • Cardiovascular Physiology
    • Cardiac Electrophysiology

    Background:

    • The sino-atrial (SA) node generates the heart's electrical impulse.
    • The inward pacemaker current (ih) and slow inward current (isi) are critical for SA node function.

    Purpose of the Study:

    • To investigate the role of the inward pacemaker current (ih) in SA node automaticity.
    • To determine the effects of cesium ions (Cs+) on SA node pacing and currents.

    Main Methods:

    • Voltage clamp technique using two microelectrodes on rabbit SA node preparations.
    • Application of varying concentrations of Cs+ (0.25-5 mM).
    • Measurement of spontaneous pacing rate and time-dependent currents.

    Main Results:

    • Cs+ (2 mM) slightly decreased spontaneous pacing rate while strongly depressing ih at potentials negative to -60 mV.

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  • Cs+ had minimal effect on other time-dependent currents.
  • Adrenaline increased pacing rate and enhanced isi without affecting Cs+-blocked ih.

    • Conclusions:

    • ih plays a minor role in SA node pacemaker depolarization.
    • isi appears to play a more prominent role in diastolic depolarization in SA nodal cells.