Stability of further-attenuated measles vaccines

Related Experiment Video

Updated: Nov 4, 2026

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo
12:42

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

Published on: January 7, 2019

G F Mann, L M Allison, J S Lloyd

    Reviews of Infectious Diseases
    |May 1, 1983
    PubMed

    Insights

    Lyophilized measles vaccines exhibit two inactivation mechanisms. A rapid, temperature-dependent loss occurs initially, followed by a consistent, first-order reaction, enabling precise vaccine stability prediction.

    Area of Science:

    • Vaccinology
    • Biophysical Chemistry
    • Pharmaceutical Stability

    Background:

    • Lyophilized measles vaccines require rigorous stability assessment.
    • Understanding virus inactivation mechanisms is crucial for predicting vaccine shelf-life.
    • Current methods may not fully capture complex inactivation kinetics.

    Purpose of the Study:

    • To elucidate the distinct mechanisms of virus inactivation in lyophilized measles vaccines.
    • To develop a predictive model for vaccine stability based on inactivation kinetics.
    • To identify a simple method for assessing vaccine quality and stability.

    Main Methods:

    • Accelerated stability testing of lyophilized measles vaccines at various temperatures.
    • Analysis of virus infectivity loss over time.
    • Application of Arrhenius kinetics to model inactivation rates.
    • Correlation of stability data with a time-temperature color indicator.

    Main Results:

    • Two primary mechanisms of measles virus inactivation were identified: a rapid, temperature-dependent phase and a subsequent first-order reaction.
    • Vaccine stability can be precisely predicted by integrating these two inactivation components.
    • Vaccines stable for one week at 37°C are predicted to remain viable for over one year at 8°C.
    • A time-temperature color indicator closely matches the inactivation reaction rate.

    Conclusions:

    • The study provides a robust framework for predicting lyophilized measles vaccine stability.
    • A simple, accelerated stability test can identify unstable vaccine products.
    • The findings support the use of time-temperature indicators for real-time vaccine quality monitoring.

    More Related Videos

    Evaluation of Host-Pathogen Responses and Vaccine Efficacy in Mice08:52

    Evaluation of Host-Pathogen Responses and Vaccine Efficacy in Mice

    Published on: February 22, 2019

    Cell-Free Scaled Production and Adjuvant Addition to a Recombinant Major Outer Membrane Protein from Chlamydia muridarum for Vaccine Development12:53

    Cell-Free Scaled Production and Adjuvant Addition to a Recombinant Major Outer Membrane Protein from Chlamydia muridarum for Vaccine Development

    Published on: March 16, 2022

    Related Concept Videos

    Vaccinations01:51

    Vaccinations

    Overview
    Immunological Memory01:23

    Immunological Memory

    Immunological memory, a pivotal pillar of the adaptive immune system, is responsible for the body's ability to remember and respond more swiftly and effectively to previously encountered pathogens. This remarkable feature is what makes vaccines so effective in preventing diseases.
    What is Immunological Memory?
    Immunological memory is an integral function of the immune system that allows it to recognize and react more rapidly and effectively to pathogens previously encountered. This feature is...
    Vaccine Production01:23

    Vaccine Production

    Vaccine production involves a sequence of upstream and downstream processes to generate a safe and effective immunological product. It begins with cultivating microorganisms, such as viruses or bacteria, to obtain antigenic material. For viral vaccines, mammalian host cells are grown in bioreactors and subsequently infected with the target virus. The virus replicates within the host cells, which are lysed to release viral particles. This lysate is then clarified through filtration or...
    Vaccines01:21

    Vaccines

    Vaccines are among the most effective tools in preventive medicine, designed to prepare the immune system to recognize and combat infectious agents. By introducing antigens—substances that the immune system identifies as foreign—vaccines stimulate an adaptive immune response that leads to immunological memory. This immunological memory enables the body to mount a faster and more effective response upon future exposures to the actual pathogen.Vaccines can be categorized based on the type of...
    Smallpox01:24

    Smallpox

    Smallpox is a severe contagious disease caused by the Variola major virus, a double-stranded DNA member of the Poxviridae family.Variola major transmission occurs primarily via inhalation of virus-laden droplets or direct contact with infectious scabs. The incubation period averages approximately seven days, although it may range from 7 to 17 days depending on the inoculum and host factors.Clinically, the prodromal phase is marked by an abrupt onset of high fever, malaise, headache, and myalgia.
    Poliomyelitis01:17

    Poliomyelitis

    Poliomyelitis is caused by poliovirus, a small, non-enveloped, positive-sense RNA virus of the Picornaviridae family and Enterovirus genus. Transmission occurs primarily via the fecal-oral route, often through ingestion of contaminated water or food. The virus initially replicates in the oropharynx and intestinal mucosa, particularly in lymphoid tissues such as the tonsils, Peyer’s patches, and regional lymph nodes. Primary viremia follows, allowing dissemination throughout the body.In most...