Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An inverted repeat borders a fivefold amplification in satellite DNA.

V Bonnewell, R F Fowler, D M Skinner

    Science (New York, N.Y.)
    |August 26, 1983
    PubMed
    Summary

    A Bermuda land crab satellite DNA variant shows significant length variation due to amplified DNA sequences. This amplification involves a tandem repeat and inverted repeat structure, differing from other organisms where DNA is inserted.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Distance and direction, but not light cues, support response reversal learning.

    Learning & behavior·2018
    Same author

    Proteins of Crustacean Exoskeletons: I. Similarities and Differences among Proteins of the Four Exoskeletal Layers of Four Brachyurans.

    The Biological bulletin·2018
    Same author

    Carroll M. Williams.

    The Biological bulletin·2018
    Same author

    Combined hippocampal and amygdala lesions block learning of a response-independent form of occasion setting.

    Behavioral neuroscience·2001
    Same author

    The moving fire hydrant experiment: movement of objects to a new location reelicits marking in rats.

    Neurobiology of learning and memory·2001
    Same author

    A two-platform task reveals a deficit in the ability of rats to return to the start location in the water maze.

    Behavioral neuroscience·2001

    Area of Science:

    • Genomics
    • Molecular Biology
    • Evolutionary Genetics

    Background:

    • Satellite DNA plays a role in genome evolution and organization.
    • Variations in satellite DNA repeat units can arise through amplification or deletion events.
    • Understanding these variations provides insights into genome plasticity.

    Purpose of the Study:

    • To characterize a significantly longer variant of Bermuda land crab satellite DNA.
    • To investigate the molecular mechanisms underlying the observed length variation in satellite DNA.
    • To compare the structural features of this variant with other satellite DNA sequences.

    Main Methods:

    • Comparative analysis of satellite DNA sequences from Bermuda land crabs.
    • Identification and characterization of tandemly amplified sequences.
    • Examination of flanking repeat structures, including inverted repeats.

    Main Results:

    • One satellite DNA variant is substantially longer than the average repeat unit.
    • The increased length is due to a fivefold tandem amplification of a 0.142-kilobase sequence.
    • This amplified sequence is flanked by a tetranucleotide inverted repeat; other variants lack one arm of this repeat.

    Conclusions:

    • Tandem amplification of specific sequences within inverted repeats contributes to satellite DNA length variation.
    • The observed structural differences suggest distinct evolutionary pathways for satellite DNA variants.
    • This mechanism of amplification within repeats contrasts with insertion mechanisms seen in other organisms.

    Related Experiment Videos