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Genetic studies on the yeast Saccharomycopsis lipolytica. Inactivation and mutagenesis
Abstract:
Spontaneous mutants of Saccharomycopsis lipolytica were selected and partially characterized. Several antibiotics and antimetabolites were used for selection of spontaneous resistant mutants from Saccharomycopsis lipolytica. The frequencies of such mutants were mainly arranged between 1 X 10(-7) and 5 X 10(-6) mutants per cell. But one class of glucosamine resistant mutants (GAMRA) occurred more frequently. Among the resistant mutants different types of dominant and recessive resistant mutants could be observed. UV light was used for inactivation of cells and induction of mutants from S. lipolytica. Comparing four haploid strains only small differences were detected in sensitivity to UV light. UV light at a dosage of 135 J/m2 was applied to increase the mutant frequencies in three haploid strains. Besides auxotrophic, temperature sensitive and colony morphology mutants, some new mutant types like small colony forming mutants, red-brown coloured mutants, some new mutant types like small colony forming mutants, red-brown coloured mutants, allylalcohol, glucosamine, 2-deoxyglucose or nystatin resistant mutants, hitherto not described for S. lipolytica, were isolated and partially characterized.
Insights
Researchers identified spontaneous mutants in Saccharomycopsis lipolytica, including novel glucosamine-resistant mutants (GAMRA). UV radiation was used to induce and characterize various new mutant types in this yeast.
Area of Science:
- * Microbiology
- * Yeast genetics
- * Molecular biology
Background:
- * Saccharomycopsis lipolytica is a yeast with industrial applications.
- * Understanding its genetic variability is crucial for strain improvement.
- * Spontaneous mutation is a key driver of genetic diversity.
Purpose of the Study:
- * To select and partially characterize spontaneous mutants of Saccharomycopsis lipolytica.
- * To investigate the frequency and types of induced mutations using UV radiation.
- * To identify novel mutant phenotypes in S. lipolytica.
Main Methods:
- * Selection of spontaneous mutants using antibiotics and antimetabolites.
- * Induction of mutations using UV irradiation (135 J/m2).
- * Partial characterization of isolated mutant strains.
Main Results:
- * Spontaneous mutant frequencies ranged from 1 x 10(-7) to 5 x 10(-6).
- * Glucosamine-resistant mutants (GAMRA) occurred with higher frequency.
- * UV treatment increased mutant frequencies and yielded novel mutants (e.g., small colony, red-brown, resistant to allylalcohol, glucosamine, 2-deoxyglucose, nystatin).
Conclusions:
- * Saccharomycopsis lipolytica can generate a variety of spontaneous and UV-induced mutants.
- * Novel mutant types, including specific resistance phenotypes, were identified.
- * These findings contribute to the genetic characterization of S. lipolytica.