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An in vitro quantitative assay for tumor cell invasion
Cancer Research
|October 1, 1978
Summary
This study introduces a new in vitro assay to quantify tumor cell invasion by measuring migration through chicken embryo membranes. This method aids in understanding the mechanisms of cancer cell invasion.
Area of Science:
- Oncology
- Developmental Biology
- Biotechnology
Background:
- Tumor cell invasion is a critical step in cancer metastasis.
- Accurate quantification of invasion is essential for understanding cancer progression.
Purpose of the Study:
- To describe a novel in vitro quantitative assay for measuring tumor cell invasion.
- To utilize the chorioallantoic membrane of developing chicken embryos as a model for invasion.
Main Methods:
- Development of specialized invasion chambers using latex cylinders and chorioallantoic membranes.
- Labeling tumor cells with [125I]iododeoxyuridine for quantitative tracking.
- Incubation of chambers in tissue culture medium and monitoring cell migration over time.
Main Results:
- The assay successfully quantifies the rate of tumor cell migration through the chorioallantoic membrane.
- Viable, labeled tumor cells were detected in the surrounding medium, on the vial, and in the sponge at various time points.
Conclusions:
- The described in vitro assay provides a reliable method for studying tumor cell invasion.
- This assay can be instrumental in elucidating the complex mechanisms underlying cancer cell metastasis.