[Submicroscopic study of brown adipose tissue in young rats by the freeze-fracturing method]

The authors report the results of their ultrastructural study on the rat brown adipose tissue using the freeze-fracturing techniques. It is well known from transmission electron microscopy that lipid vacuoles of the brown adipose tissue cells are bound by a dense line corresponding to a structure as yet totally unknown. The results of the study using freeze-fracturing technics show that the morphology of lipid vacuoles surface is different from that of the typical unit membrane. The possibility is discussed that the particular aspect of the surface is due to the lypolysis induced by tissue fixation.