Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Determination of HDL-cholesterol. Comparison between two methods.

M R Averna, G Lo Cascio, G B Rini

    Bollettino Della Societa Italiana Di Biologia Sperimentale
    |April 30, 1983
    PubMed
    Summary

    A new method for High-Density Lipoprotein (HDL) Cholesterol assay using heparin-CaCl2-NaCl2 shows precise and accurate results. This method correlates well with the PTA-MgCl2 technique for measuring HDL Cholesterol levels.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Six-month humoral and cellular immune response to the third dose of BNT162b2 anti-SARS-CoV-2 vaccine in patients with solid tumors: a longitudinal cohort study with a focus on the variants of concern.

    ESMO open·2022
    Same author

    Humoral and cellular response before and after the fourth BNT162b2 vaccine dose in patients with solid tumors on active treatment.

    Annals of oncology : official journal of the European Society for Medical Oncology·2022
    Same author

    Immunogenicity and safety after the third dose of BNT162b2 anti-SARS-CoV-2 vaccine in patients with solid tumors on active treatment: a prospective cohort study.

    ESMO open·2022
    Same author

    Analysis of the humoral and cellular immune response after a full course of BNT162b2 anti-SARS-CoV-2 vaccine in cancer patients treated with PD-1/PD-L1 inhibitors with or without chemotherapy: an update after 6 months of follow-up.

    ESMO open·2022
    Same author

    A snapshot of the immunogenicity, efficacy and safety of a full course of BNT162b2 anti-SARS-CoV-2 vaccine in cancer patients treated with PD-1/PD-L1 inhibitors: a longitudinal cohort study.

    ESMO open·2021
    Same author

    Estimating COVID-19 recovery time in a cohort of Italian healthcare workers who underwent surveillance swab testing.

    Public health·2021

    Area of Science:

    • Clinical Chemistry
    • Biochemistry
    • Cardiovascular Diagnostics

    Background:

    • Accurate measurement of High-Density Lipoprotein (HDL) Cholesterol is crucial for cardiovascular risk assessment.
    • Existing methods for HDL Cholesterol assay may have limitations in precision or accuracy.
    • The development of novel, reliable assay techniques is an ongoing area of research.

    Purpose of the Study:

    • To evaluate a new method for quantifying HDL Cholesterol.
    • To assess the precision and accuracy of the novel heparin-CaCl2-NaCl2 assay.
    • To compare the performance of the new method with a standard technique (PTA-MgCl2).

    Main Methods:

    • A new HDL Cholesterol assay was developed utilizing heparin-CaCl2-NaCl2.
    • The assay's precision and accuracy were evaluated.

    Related Experiment Videos

  • Results were compared against the established PTA-MgCl2 method for HDL Cholesterol determination.

    • Main Results:

    • The heparin-CaCl2-NaCl2 method demonstrated good correlation with the PTA-MgCl2 technique.
    • The assay yielded precise and accurate HDL Cholesterol measurements.
    • Mean HDL-C values were determined for normolipemic and hypertriglyceridemic subjects.

    Conclusions:

    • The novel heparin-CaCl2-NaCl2 method is a precise and accurate technique for HDL Cholesterol assay.
    • This method offers a reliable alternative for routine clinical laboratory use.
    • Further validation in diverse patient populations may be warranted.