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[Evaluation of ion activity in determining time of death]

Iu L Mel'nikov, A S Zhakenov, V F Antonov

Biulleten' Eksperimental'Noi Biologii I Meditsiny

|August 1, 1983

Abstract:

Activity of potassium ions was examined in 3 liquid media of 54 rabbit cadavers by potentiometry with the use of a potassium-selective valinomycin electrode. The reported and obtained data were used for the development of the diagnostic regression equations and the curves to define the time of death from the activity of a complex of 3 ions (potassium, sodium and hydrogen) in the blood serum, CSF and vitreous body fluid. The method is offered for appropriate trials in human cadavers.

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Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a result, EDTA...

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