Separation of estrogen receptors from nonspecific binding employing receptor antibodies

Sedimentation analysis in the presence of monoclonal antibodies to the estrogen receptor results in a well-marked separation of the estrogen receptor peak from that containing significant amounts of nonspecific binding. This method has been applied to the study of liver and breast cancer cytosols. These findings are of importance in the study of breast cancer metastases and of endocrine-responsive organs such as the liver, that previously could not be readily analyzed because of the presence of significant amounts of nonspecific binding.