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Related Experiment Videos

Coelomocyte motility.

K T Edds, C Chambers, R D Allen

    Cell Motility
    |January 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Coelomocytes exhibit fibroblast-like movement in coelomic fluid, extending lamellipodia and filopodia. When isolated, these cells lose motility but their filopodia show rapid waving and bending motions.

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    Area of Science:

    • Cell Biology
    • Biophysics

    Background:

    • Coelomocytes are immune cells found in the coelomic fluid of certain invertebrates.
    • Understanding coelomocyte motility is crucial for comprehending immune responses and cellular dynamics.

    Purpose of the Study:

    • To investigate the motility of coelomocytes and their filopodia using advanced microscopy.
    • To differentiate between cell motility in native coelomic fluid versus an artificial buffer.

    Main Methods:

    • Utilized a video-enhanced contrast system with a differential interference contrast (DIC) microscope.
    • Observed whole coelomocytes and individual filopodia under varying fluid conditions.

    Main Results:

    • Coelomocytes in diluted coelomic fluid displayed fibroblast-like translocation, extending lamellipodia and retraction fibers.

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  • Isolated coelomocytes in an isotonic buffer lost translocation ability, but their filopodia exhibited rapid waving and bending.
  • Conclusions:

    • Coelomic fluid is essential for coelomocyte translocation.
    • Filopodia display intrinsic dynamic behaviors, including folding and re-extension, even when cell body translocation is inhibited.