Related Experiment Video
Updated: Oct 1, 2026

19:16
The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
Published on: March 17, 2010
Trypsin-pancreatic secretory isoinhibitor A from bovine pancreas (Kazal type). Spectroscopic study on structure and
Abstract:
The secondary and tertiary structure of isoinhibitor A from bovine pancreas secretion (Kazal inhibitor) was investigated by circular dichroism (CD) and fluorescence measurements. The protein shows noteworthy thermal stability as seen by the temperature dependence of the CD spectra and the intensity of emission fluorescence at different pH values.

