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Quantitative thin layer chromatographic multi-sulfonamide screening procedure: collaborative study.

M H Thomas, R L Epstein, R B Ashworth

    Journal - Association of Official Analytical Chemists
    |July 1, 1983
    PubMed
    Summary
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    A validated thin-layer chromatography method accurately detects multiple sulfonamides at 0.1 ppm in animal tissues. This study confirms high average recovery rates and reliable precision for sulfonamide analysis in food safety.

    Area of Science:

    • Analytical Chemistry
    • Food Safety
    • Veterinary Pharmacology

    Background:

    • Sulfonamides are widely used antimicrobial agents in food-producing animals.
    • Accurate detection of sulfonamide residues is crucial for ensuring food safety and preventing antimicrobial resistance.
    • Existing analytical methods may lack the sensitivity or throughput for comprehensive screening.

    Purpose of the Study:

    • To evaluate a thin-layer chromatographic (TLC) procedure for the simultaneous detection of multiple sulfonamides.
    • To determine the method's suitability for analyzing sulfonamide residues in various animal tissues.
    • To assess the method's performance through an interlaboratory collaborative study.

    Main Methods:

    • Thin-layer chromatography (TLC) was employed for sulfonamide separation and detection.

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  • The method was validated for detecting sulfamethazine, sulfadimethoxine, and sulfaquinoxaline.
  • Analysis was performed on liver and muscle tissues from swine, turkey, and duck.
  • An interlaboratory collaborative study was conducted to assess method robustness.

    • Main Results:

    • The TLC method demonstrated suitability for detecting sulfonamides at a concentration of 0.1 ppm.
    • Average recovery rates across all tested drugs and tissues were high, averaging 95%.
    • The method exhibited good precision, with repeatability at 7.7% and reproducibility at 10.5%.

    Conclusions:

    • The developed TLC procedure is a reliable and sensitive method for the simultaneous analysis of multiple sulfonamide residues in animal tissues.
    • The method's high recovery and precision support its application in routine food safety monitoring.
    • This validated method contributes to ensuring the safety of animal-derived food products.