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Automated cytopathic effect (CPE) assays.

W J McAleer, W J Miller, W M Hurni

    Journal of Biological Standardization
    |July 1, 1983
    PubMed
    Summary
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    This study introduces an automated cell-based assay for titrating measles, mumps, and rubella viruses in vaccines. The new method enhances precision and efficiency for vaccine manufacturers.

    Area of Science:

    • Virology
    • Vaccinology
    • Biotechnology

    Background:

    • Accurate titration of viral antigens is crucial for vaccine quality control.
    • Traditional methods for cytopathic effect (CPE) assays can be labor-intensive and prone to variability.
    • Measles, mumps, and rubella (MMR) virus quantification requires precise and reproducible techniques.

    Purpose of the Study:

    • To develop and validate an automated procedure for performing cytopathic effect (CPE) titrations.
    • To improve the precision, ease of use, and cost-effectiveness of viral titration in vaccine materials.
    • To provide a more efficient method for quantifying measles, mumps, and rubella viruses.

    Main Methods:

    • An automated system utilizing a modified Dynatiter instrument and 96-well microtitre plates was employed.

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  • Automated addition of cell suspensions and reagents, along with sample dilution.
  • Carbolfuchsin staining of cell monolayers to obviate microscopic examination.
  • Automated endpoint determination using an optical scanner and programmable calculator.

    • Main Results:

    • The automated CPE procedure significantly increased the precision and ease of viral titrations.
    • Elimination of manual microscopic examination reduced potential for human error.
    • Automated data processing ensured accurate endpoint calculation.
    • The method demonstrated value for vaccine manufacturers through increased accuracy and precision at reasonable cost.

    Conclusions:

    • The developed automated CPE procedure offers a more precise and efficient method for titrating MMR viruses in vaccines.
    • This automation streamlines the assay process, reducing labor and enhancing reproducibility.
    • The enhanced accuracy and cost-effectiveness make this method highly beneficial for vaccine manufacturers and quality control.