Immunoreactive prolactin in subcellular fractions from bovine mammary tissue

Mammary tissue from lactating Holsteins at slaughter was homogenized and fractionated into subcellular compartments. Bovine prolactin was quantified in each subcellular fraction and in preslaughter milk by radioimmunoassay procedures validated for this purpose. Prolactin concentrations were ng prolactin/mg protein for all samples. Immunoreactive prolactin was in rough endoplasmic reticulum (24.8 ng/mg), Golgi apparatus (18.8 ng/mg), and secretory vesicle (10.2 ng/mg). All of these concentrations exceeded that in samples of milk (2.7 ng/mg). Nuclear fractions contained a material that reacted in the prolactin radioimmunoassay, but criteria for valid quantification could not be satisfied. These results confirm prolactin inside bovine mammary cells, and they are consistent with an intracellular pathway for transfer of prolactin from plasma into milk.