Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Enzymic serum cholesterol measurement with a basic autoanalyzer and Du Pont aca method.

Clinical Chemistry

|September 1, 1978

Summary

This study compared two methods for measuring total serum cholesterol. The continuous-flow system showed less interference from bilirubin and lipemia than the discrete analyzer, offering more reliable cholesterol results.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

EMG activity of medial and lateral hamstrings at three positions of tibial rotation during low-force isometric knee flexion contractions.

Journal of back and musculoskeletal rehabilitation·2014

Same author

Autoradiographic studies of oral lichen planus.

Oral surgery, oral medicine, and oral pathology·1982

Same author

Automation of the homogeneous substrate-labeled fluorescent immunoassay for theophylline in serum: application to the determination of phenytoin and phenobarbital in serum.

Therapeutic drug monitoring·1982

Same author

An animal model for the study of prostatic adenocarcinoma.

Investigative urology·1977

Same author

Observations on possible autoimmune therapeutic effects in experimentally produced rat bladder tumors.

Urologia internationalis·1975

Same author

Tissue metabolism: effect of a mucopolysaccharide cream.

Arzneimittel-Forschung·1974

Area of Science:

Clinical Chemistry
Analytical Chemistry
Biochemistry

Background:

Accurate measurement of total serum cholesterol is crucial for cardiovascular risk assessment.
Enzymic methods are commonly used for cholesterol determination.
Different analytical platforms may exhibit varying performance characteristics.

Purpose of the Study:

To compare the performance of discrete (Du Pont aca) and continuous-flow (Technicon AutoAnalyzer I) enzymic methods for total serum cholesterol analysis.
To evaluate the impact of icterus and lipemia on cholesterol measurements by these methods.

Main Methods:

Enzymic methods for total serum cholesterol were employed.
Analysis was performed on normal, icteric, and lipemic sera.
Discrete (Du Pont aca) and continuous-flow (Technicon AutoAnalyzer I) systems were utilized.

Main Results:

A regression equation was established for clear, non-icteric sera: y (continuous flow) = 0.944x (aca) + 10.69 (r = 0.971).
The continuous-flow system showed no significant interference from bilirubin up to 350 mg/L.
The aca method exhibited interference from bilirubin and falsely elevated cholesterol values in lipemic sera, with values increasing proportionally to triglyceride concentration.

Conclusions:

The continuous-flow system demonstrated superior performance regarding interference from bilirubin and lipemia compared to the discrete aca method.
The AutoAnalyzer procedure provided more reliable total serum cholesterol measurements in the presence of common interfering substances.
Clinical laboratories should consider the potential interferences when selecting an analytical platform for cholesterol testing.

Related Experiment Videos