Related Experiment Videos
In vivo correlation between stratum corneum reservoir function and percutaneous absorption
The Journal of Investigative Dermatology
|September 1, 1983
Summary
Stratum corneum reservoir function predicts percutaneous absorption in rats. Measuring horny layer concentration accurately forecasts substance penetration through the skin.
Area of Science:
- Pharmacokinetics
- Dermatology
- Toxicology
Background:
- Percutaneous absorption is influenced by stratum corneum reservoir function.
- Understanding this relationship is crucial for drug delivery and toxicology assessments.
Purpose of the Study:
- To establish a quantitative relationship between stratum corneum reservoir function and subsequent percutaneous absorption.
- To determine if stratum corneum concentration can predict the total systemic distribution of topically applied substances.
Main Methods:
- Topical application of 10 diverse substances (200 nmol) to hairless rats for 30 minutes.
- Quantification of stratum corneum reservoir concentration via tape-stripping and liquid scintillation counting.
- Measurement of total body distribution and excreta after 96 hours.
Main Results:
- A strong linear correlation (r=0.998) was found between stratum corneum concentration (x) and systemic distribution (y): y = 1.644x - 0.536.
- 80-95% of applied substances were excreted in urine, with rapid elimination for most compounds.
- A 50-fold difference in skin penetration was observed between benzoic acid and dexamethasone.
Conclusions:
- Stratum corneum concentration serves as a reliable predictor of percutaneous absorption and systemic distribution.
- This predictive model can be utilized to estimate the amount of substance penetrating intact rat skin.
- Further validation in human subjects is recommended to confirm these findings.