Recovery of distal changes after nerve constriction by a ligature

When ligatures were used to constrict the proximal tibial nerve in rabbits, nerve fibres distal to the site of constriction underwent a reduction in axonal and external fibre diameter, and in conduction velocity. Distal changes could be detected within 7-12 days of the onset of constriction; removal of the ligatures was followed by partial recovery. When paranodal myelin damage occurred in the distal tibial nerve, it showed the typical non-random distribution of secondary demyelination, with multiple paranodal defects on some fibres and complete sparing of others. These findings emphasize the role of the axon in the genesis of paranodal demyelination.