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Through-the-Wall Blood Sampling Method to Minimize Sleep Disruption in Clinical Settings06:39

Through-the-Wall Blood Sampling Method to Minimize Sleep Disruption in Clinical Settings

Here, we present a through-the-wall, minimally invasive method to sample blood during sleep in clinical...
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Soxhlet Extraction of Lipid Biomarkers from Sediment08:04

Soxhlet Extraction of Lipid Biomarkers from Sediment

Source: Laboratory of Jeff Salacup - University of Massachusetts Amherst
Every lab needs standards that track the performance, accuracy, and precision of its instruments over time to ensure a measurement made today is the same as a measurement made a year from now (Figure 1). Because standards must test the performance of instruments over a long period of time, large volumes of the standards are often required. Many chemical standards can be purchased from retail scientific companies, like...
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Sonication Extraction of Lipid Biomarkers from Sediment07:24

Sonication Extraction of Lipid Biomarkers from Sediment

Source: Laboratory of Jeff Salacup - University of Massachusetts Amherst
The material comprising the living "organic" share of any ecosystem (leaves, fungi, bark, tissue; Figure 1) differs fundamentally from the material of the non-living "inorganic" share (rocks and their constituent minerals, oxygen, water, metals). Organic material contains carbon linked to a series of other carbon and hydrogen molecules (Figure 2), which distinguishes it from inorganic material. Carbon's wide valency range...
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Measuring Protein Binding to F-actin by Co-sedimentation06:17

Measuring Protein Binding to F-actin by Co-sedimentation

This protocol describes a method to test the ability of a protein to co-sediment with filamentous actin (F-actin) and, if binding is observed, to measure the affinity of the...
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Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay14:45

Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay

In this report, we demonstrate the staining and analysis steps of a phenotyping assay performed on fresh whole blood to enumerate major innate and adaptive leukocyte populations. We emphasize considerations for performing these procedures in the context of a multicenter clinical...
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Pre-Procedural Guidelines for Assessing Blood Pressure01:10

Pre-Procedural Guidelines for Assessing Blood Pressure

Accurate blood pressure assessment is crucial for diagnosing and managing various health conditions. To ensure the reliability of these measurements, healthcare professionals must adhere to standardized pre-procedural guidelines. These guidelines enhance patient safety and improve the overall quality of healthcare. The following steps are essential for obtaining accurate and consistent blood pressure readings, from using the appropriate tools to ensuring effective communication with the...
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