[Testosterone concentrations in human biological fluids following various routes of administration]

The antiserum against testosterone (T) was obtained and its titer-dependent specificity was studied during the rabbit immunization. The radioimmunoassay for T determination in the human saliva, blood serum and urine was developed. The sensitivity is 6 pg, the variation coefficient within the experiment is 5.3 and between experiments is 10.5%, the standard confirmation is 95 to 100% and dihydrotestosterone cross reaction is 62%. The T content in human biological liquids was studied after various routes of its administration. The highest steroid concentration in the blood is seen 0.5 and 2 hours after the oral and intramuscular administration, exceeding the initial level 2-3 times. The highest T concentrations in the saliva and urine are observed 0.5 and 2, 2 and 24 hours after the oral and intramuscular use, respectively, the levels being 40 to 90 times (oral administration) and 2-3 fold (intramuscular injections) highest versus the initial ones. The changes in the saliva T content indicate the T level in the blood. It was shown that the free and total T levels in sportswomen urine are markedly higher in comparison with those of the females, not going in for sport. The T content in the sportsmen urine is within the limits, characteristic for healthy males.