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Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes
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The response to insulin by adipose tissue of insulin-dependent diabetics in vitro

B Schultz, S Knospe, P Heinke

    Endokrinologie
    |March 1, 1978
    PubMed

    Subcutaneous adipose tissue was removed by surgical biopsy from 10 normal subjects and 4 insulin-dependent diabetics under exact intracutaneous anaesthesia. The adipose tissue fragments or isolated fat cells were incubated with a tracer amount of labeled glucose. In the presence of insulin (62.5 muU/ml) the production of carbon dioxide from glucose by adipose tissue fragments of nonobese controls as well as diabetics increased up to 286 +/- 62% and 198 +/- 14.7%, respectively. Furthermore, triglyceride synthesis was raised to 257 +/- 30% and 267 +/- 34%, respectively. There were no differences in the sensitivity of adipose tissue to insulin of healthy volunteers and juvenile diabetics.

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