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Lipid-bound oligosaccharides in insects.

L A Quesada Allue, E Belocopitow

    European Journal of Biochemistry
    |August 1, 1978
    PubMed
    Summary

    Fruit fly membranes transfer mannose to create lipid-linked oligosaccharides, essential for glycoprotein synthesis. These compounds, polyprenyl derivatives, are key intermediates in the insect

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    Area of Science:

    • Biochemistry
    • Glycobiology
    • Insect Molecular Biology

    Background:

    • Oligosaccharide synthesis is crucial for glycoprotein formation.
    • Lipid-linked oligosaccharides (LLOs) are key intermediates in this process.
    • Understanding LLO synthesis in insects provides insights into conserved biological pathways.

    Purpose of the Study:

    • To investigate the synthesis of lipid-linked oligosaccharides in the fruit fly Ceratitis capitata.
    • To characterize the initial steps of mannose and N-acetylglucosamine transfer into LLOs.
    • To explore the potential role of these LLOs in glycoprotein biosynthesis.

    Main Methods:

    • Enzymatic assays using GDP-[14C]mannose and UDP-N-acetyl[14C]glucosamine with insect membrane preparations.
    • Characterization of LLOs via mild acid hydrolysis and analysis of their structure.
    • Investigating the effect of an acidic glycolipid fraction on LLO formation.

    Main Results:

    • Membrane preparations catalyze mannose transfer from GDP-mannose into polyprenyl-pyrophosphate-oligosaccharides.
    • A trisaccharide lipid, polyprenyl-pyrophosphate-N,N'-diacetylchitobiose-mannose, was partially characterized.
    • Synthesis of higher LLOs was observed upon addition of unlabeled GDP-mannose or UDP-N-acetylglucosamine, indicating sequential assembly.
    • An acidic glycolipid fraction stimulated LLO formation.

    Conclusions:

    • Ceratitis capitata membranes synthesize polyprenyl-pyrophosphate-oligosaccharides, analogous to pathways in other organisms.
    • The characterized trisaccharide lipid is a precursor for higher oligosaccharide structures.
    • These findings suggest a role for insect polyprenyl oligosaccharides in glycoprotein biosynthesis.

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