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Suppression of CFU-s activity by rabbit-anti-mouse brain serum can be overcome by treatment with papain
Experimental Hematology
|August 1, 1978
Abstract:
The suppression of CFU-s activity which is observed after incubation with rabbit-anti-mouse brain sera (RAMB) can be reversed by incubating the antibody coated cells with papain. Papain treatment does not alter the CFU-s membrane since pre-treatment with the enzyme does not protect CFU-s from the lethal effect of RAMB. This technique should allow the use of fluoresceinated or otherwise labelled RAMB for the selection of CFU-s.
Insights
Rabbit-anti-mouse brain sera (RAMB) suppress CFU-s activity, but papain treatment reverses this effect. This method enables the selection of CFU-s using labeled RAMB.
Area of Science:
- Hematology
- Immunology
- Cell Biology
Background:
- Colony-forming unit-spleen (CFU-s) are crucial hematopoietic stem cells.
- Rabbit-anti-mouse brain sera (RAMB) can suppress CFU-s activity.
- Understanding CFU-s regulation is vital for stem cell research.
Purpose of the Study:
- To investigate methods for reversing RAMB-induced suppression of CFU-s activity.
- To determine if papain treatment can restore CFU-s function after RAMB exposure.
- To assess the feasibility of using this technique for CFU-s selection.
Main Methods:
- Incubation of CFU-s with RAMB.
- Treatment of antibody-coated CFU-s with papain.
- Assessment of CFU-s activity post-treatment.
- Pre-treatment of CFU-s with papain followed by RAMB exposure.
Main Results:
- Papain treatment reversed the suppressive effect of RAMB on CFU-s activity.
- Papain did not alter the CFU-s membrane integrity.
- Pre-treatment with papain did not protect CFU-s from RAMB's lethal effects.
Conclusions:
- Papain treatment offers a method to restore RAMB-affected CFU-s activity.
- The CFU-s membrane remains intact after papain treatment.
- This technique facilitates the use of labeled RAMB for CFU-s selection and isolation.