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Involvement of microfilaments in filopodial function of phagocytic granular cells of the silkworm, Bombyx mori
Abstract:
The effects of colchicine, vinblastine, and cytochalasin B (CB) on both attachment and filopodial function of phagocytic granular cells of Bombyx mori were studied using the scanning electron microscopy (SEM). Incubation of cells with colchicine or vinblastine had only a little effect on attachment and filopodial function. CB, on the other hand, caused a marked inhibition of filopodia elongation and thus inhibited attachment. The effects of all drugs were dose-dependent. Colchicine and vinblastine did not affect filopodia contractility. Treatment of cells with CB, however, caused retraction of once-elongated filopodia. The inhibitory effect of CB on elongation was reversible with treatment of hemolymph. The results strongly suggest that cytochalasin-sensitive structures, most likely microfilaments, are involved in filopodial function and that hemolymph will be of great importance in the activation of microfilament system.
Insights
Cytochalasin B significantly inhibits filopodial elongation and cell attachment in Bombyx mori granular cells. This effect is reversible by hemolymph, suggesting microfilaments are crucial for cell function.
Area of Science:
- Cell Biology
- Biochemistry
- Insect Physiology
Background:
- Phagocytic granular cells in Bombyx mori play a vital role in the insect immune response.
- Understanding the mechanisms of cell attachment and filopodial function is crucial for comprehending cellular motility and immune surveillance.
Purpose of the Study:
- To investigate the impact of colchicine, vinblastine, and cytochalasin B on the attachment and filopodial dynamics of Bombyx mori phagocytic granular cells.
- To elucidate the role of microfilaments in filopodial function and cell attachment.
Main Methods:
- Scanning electron microscopy (SEM) was employed to visualize and analyze cellular structures and functions.
- Dose-dependent effects of colchicine, vinblastine, and cytochalasin B on granular cells were assessed.
- Reversibility of drug-induced effects using hemolymph was examined.
Main Results:
- Colchicine and vinblastine exhibited minimal effects on cell attachment and filopodial function.
- Cytochalasin B markedly inhibited filopodia elongation and attachment in a dose-dependent manner.
- Cytochalasin B induced retraction of elongated filopodia, an effect reversible by hemolymph treatment.
Conclusions:
- Cytochalasin-sensitive structures, presumed to be microfilaments, are essential for filopodial elongation and cell attachment in Bombyx mori granular cells.
- Hemolymph plays a significant role in activating the microfilament system, influencing cellular functions.