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Natural agglutinins to hidden membrane components in the bovine erythrocytes

J Sellei

    Journal of Immunogenetics
    |December 1, 1982
    PubMed

    Digestion of bovine erythrocytes (BE) with trypsin (Tr) or chymotrypsin (CTr) revealed 'Tr and CTr specific' receptors respectively. These receptors reacted with all the isologous, including the autologous, sera. The titres of agglutinability varied for the Tr-digested BE from 1:2 to 1:64 and from zero to 1:128 for the CTr-digested BE. However, the variation between the members of monozygous twin pairs (MZ) in no case exceeded +/- 2 score units, the error limits. Agglutination of the CTr-digested BE gave with the PHA lectin very similar, (r = 0.964) results to that which was obtained with the isologous sera. The titre of agglutinins showed concordance in all the sera of MZ pairs when tested against the neuraminidase-digested human erythrocytes and the Tr or CTr-digested BE. Their variance due to differences varied between MZ pairs from 60.4 to 74.3%.

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