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Related Experiment Videos

Intrathecal morphine in mice: a new technique.

J L Hylden, G L Wilcox

    European Journal of Pharmacology
    |October 17, 1980
    PubMed
    Summary

    Researchers developed a quick method for intrathecal injections in mice. This technique confirmed that intrathecal morphine causes analgesia and Straub tail response, effects blocked by naloxone.

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    Area of Science:

    • Neuroscience
    • Pharmacology
    • Animal Models

    Background:

    • Intrathecal drug administration is crucial for studying central nervous system effects.
    • Developing efficient and reliable methods for intrathecal injections is essential for preclinical research.

    Purpose of the Study:

    • To describe a simple, rapid technique for intrathecal injections in unanesthetized mice.
    • To investigate the distribution and effects of intrathecally administered morphine in mice.

    Main Methods:

    • A lumbar puncture technique was employed for intrathecal injections in conscious mice.
    • Distribution of intrathecal [3H]morphine was assessed in brain regions.
    • Behavioral responses (Straub tail, tail-flick) and dose-dependency were evaluated.
    • Antagonism by naloxone was examined.

    Main Results:

    • The described technique allowed for rapid intrathecal injections.
    • Significant [3H]morphine levels were not detected in the midbrain or forebrain after intrathecal administration.
    • Submicrogram doses of morphine sulfate induced dose-related Straub tail and tail-flick analgesia.
    • Naloxone effectively antagonized morphine-induced effects.

    Conclusions:

    • The developed method is effective for intrathecal drug delivery in mice.
    • Intrathecal morphine exerts central analgesic effects and induces specific behavioral responses.
    • These effects are mediated via opioid receptors, as evidenced by naloxone antagonism.

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