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Updated: Feb 25, 2026
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Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates
Published on: May 29, 2018
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Chimaeric rabbits from immunosurgically-prepared inner-cell-mass transplantation
Journal of Embryology and Experimental Morphology
|December 1, 1980
Summary
Rabbit inner cell masses (ICMs) were isolated and shown to retain developmental potential. These ICMs can form chimeric rabbits when introduced into host blastocysts, demonstrating their viability and contribution to development.
Area of Science:
- Developmental biology
- Reproductive science
- Animal genetics
Background:
- The inner cell mass (ICM) is crucial for embryonic development.
- Understanding ICM developmental potential is key for reproductive technologies.
Purpose of the Study:
- To prepare and characterize rabbit inner cell masses (ICMs).
- To assess the developmental potential of immunosurgically isolated rabbit ICMs.
- To determine if rabbit ICMs can contribute to chimaeric embryo development.
Main Methods:
- Isolation of rabbit ICMs from blastocysts using immunosurgery.
- Injection of isolated ICMs into host blastocysts.
- Implantation of composite blastocysts into foster mothers.
- Chimaerism assessment using immunoglobulin allotypic markers and progeny testing.
Main Results:
- Rabbit ICMs were successfully prepared via immunosurgery.
- Prepared ICMs retained their developmental potential.
- Composite blastocysts developed into chimaeric rabbits at a high rate.
- Chimaerism was confirmed through genetic markers and progeny analysis.
Conclusions:
- Immunosurgery is an effective method for isolating rabbit ICMs.
- Rabbit ICMs maintain their developmental capacity and can contribute to chimaeric development.
- This study provides a foundation for further research in rabbit developmental biology and chimaera generation.
