Distribution, quantification and biological activity of messenger RNA coding for human chorionic somatomammotropin...

Synthesis of hCS by RNA fractions from human placentas obtained at different stages of pregnancy was estimated either by immunological or electrophoretical methods in wheat-germ and reticulocyte cell-free systems. hCS synthesis is preferentially associated with polyribosomes bound to the membrane of the endoplasmic reticulum, as is assumed for a secreted protein. In both translational systems we determined only one precursor form of this hormone of a molecular weight near 24 000. Full-term placentas synthesize hCS as the major protein. This conveniently allowed us to isolate the messenger RNA coding for the hormone and to synthesize a specific hCS complementary DNA which we used as a probe for quantifying sequences of RNA coding for hCS during pregnancy. In placentas from first-trimester pregnancy, the concentration of hCS mRNA was 4 times less than in the full-term organs, and the hCS synthesis per microgram of RNA added into the translational medium was diminished in the same order of magnitude. In placentas from second-trimester pregnancy, the concentration of hCS mRNA was similar to that obtained at term, and in vitro the hCS synthesis per microgram of translated RNA was also similar to that observed at the end of pregnancy. However, the hCS mRNA content per placenta from mid-term pregnancy was much lower than from full-term gestation. We established a good parallelism, as pregnancy progressed, between the hCS mRNA content, its capacity of hCS synthesis in vitro and the maternal plasma hCS level, indicating that hCS production is controlled essentially by the biological active mass of the placenta.