Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fluorescently labeled myosin subfragment 1: identification of the kinetic step associated with the adenosine

D J Marsh, L A Stein, E Eisenberg

    Biochemistry
    |April 13, 1982
    PubMed
    Summary

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Effects of Radurization and Refrigerated Storage on Quality and Shelf-Life of Freshwater Prawns, Macrobrachium rosenbergii <sup>1</sup>.

    Journal of food protection·2019
    Same author

    Standards for the diagnosis and management of complex regional pain syndrome: Results of a European Pain Federation task force.

    European journal of pain (London, England)·2019
    Same author

    Medical cannabis: A forward vision for the clinician.

    European journal of pain (London, England)·2018
    Same author

    Amputation in patients with complex regional pain syndrome: a comparative study between amputees and non-amputees with intractable disease.

    The bone & joint journal·2016
    Same author

    Using a multimedia presentation to improve patient understanding and satisfaction with informed consent for minimally invasive vascular procedures.

    The surgeon : journal of the Royal Colleges of Surgeons of Edinburgh and Ireland·2015
    Same author

    Who can benefit from virtual reality to reduce experimental pain? A crossover study in healthy subjects.

    European journal of pain (London, England)·2015

    Researchers studied myosin subfragment 1 (S-1) using a fluorescence label. MgATP binding causes a fluorescence decrease, primarily due to ATP hydrolysis, indicating a conformational change in S-1.

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Muscle Contraction

    Background:

    • Myosin subfragment 1 (S-1) is crucial for muscle contraction.
    • Previous studies labeled S-1's alkali 1 light chain with 5-(iodoacetamido)fluorescein (IAF).
    • MgATP binding to labeled S-1 caused a fluorescence decrease.

    Purpose of the Study:

    • Investigate the kinetics of fluorescence decrease upon MgATP binding to labeled S-1.
    • Determine the relationship between ATP hydrolysis and fluorescence changes.

    Main Methods:

    • Utilized pre-steady-state kinetic analysis.
    • Measured fluorescence changes of the IAF label on S-1.
    • Studied the effect of MgATP binding and hydrolysis.

    Main Results:

    Related Experiment Videos

    • The decrease in IAF fluorescence is predominantly linked to the ATP-hydrolysis step.
    • This suggests a conformational alteration in S-1 during ATP hydrolysis.
    • The microenvironment of the IAF fluorophore changes due to ATP hydrolysis.

    Conclusions:

    • ATP hydrolysis induces significant conformational changes in myosin subfragment 1.
    • These conformational changes directly impact the microenvironment of the bound IAF label.
    • The study provides insights into the molecular mechanisms of muscle contraction.